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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial role in endorsing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two added splice variants had been claimed but thorough nucleotide sequence info will not be but available.
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Blocking peptides are peptides that bind precisely to your target antibody and block antibody binding. These peptide commonly is made up of the epitope regarded from the antibody.
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This guideline outlines even more feasible optimization of mobile and immunoassay parameters to make sure the very best final results are...
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This guide outlines even further attainable optimization of cellular and immunoassay parameters to be certain the very best results are...
Antibodies sure to the blocking peptide now not bind on the epitope on the concentrate on protein. By comparing the staining from your blocked antibody as opposed to the antibody by yourself, you can see which staining is unique.
Focus on Info Cyclin E belongs into the really conserved cyclin family members, whose associates are characterized by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins show unique expression and degradation designs which contribute to the temporal coordination of each and every mitotic event. Cyclin E types a complex with and functions like a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates within the G1-S section boundary and is also degraded as cells development as a result of S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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